个人简介

基本信息：制药工程专业。职称：教授；邮箱：mindong@tju.edu.cn

研究方向：合成生物学、化学生物学

个人介绍：本科、硕士毕业于天津大学，博士毕业于北京大学。香港大学和美国康奈尔大学历任博士后，助理研究员。2019年加入天津大学。入选国家级人才计划青年项目，天津市海外高层次人才青年项目，天津大学北洋青年学者。

研究领域

1.金属酶催化的自由基反应的发现、机制研究和功能改造

2.天然产物生物合成和生物氟化

3.辅因子S-腺苷甲硫氨酸相关化学生物学和合成生物学研究

4.酶抑制剂设计和药物研发

学术成就

主要从事天然产物生物合成与酶学研究，重点关注酶新功能发现、机制解析、活性优化及非天然酶促反应发现。近年来在Science、JACS、Angew. Chem. Int. Ed.、ACS Catalysis等期刊发表学术论文多篇。主持完成国家自然科学基金和天津市自然基金等十余项。

代表性文章

1. Zhen. X.; Chen, F.; Dong, M.* .Biosynthesis of Methylazetidinecarboxylic Acid with Opposite Stereoconfigurations in Vioprolides and Bonnevillamides. ACS Catalysis. 2025, 15, 18556-18562  

2. Zhao H.; Yu, N.; Wang, S.; Dong, M.*. Cyclopropylmethyl S-adenosyl-l-methionine: an enzymatic cyclopropylmethyl donor.Chem. Sci. 2025,16, 21483-21488

3. Wei, Z.; Yu, N.; Xu, T.; Liu, Z.; Li, B.; Dong, M.* Reductive Photoenzymatic O-Demethylation of Aryl Methyl Ethers and Lignin Monoaromatics Produces Methane ACS Catalysis. 2025, 15, 16, 13712–13719

4. Wu, X.; Dong, M.* Photoaffinity SAM analogues for the identification of SAM-binding proteins. Chem. Sci. 2025,16, 12043-12050

5. Kong, L.; Zhang, J.; Wang, H.; Wei, Z.; Wang, W.; Hu, J.*; Dong, M.*B12‐Dependent Radical SAM Enzymes Catalyze C‐Fluoromethylation via a CH2F‐Cobalamin Intermediate. Angew. Chem. Int. Ed. 2025, e202419815

6. Yao, Y.; He, J.; Chen, F.; Dong, M.*. Arsinothricin Biosynthesis Involving a Radical SAM Enzyme for Noncanonical SAM Cleavage and C-As Bond Formation. J. Am. Chem. Soc. 2024, 146, 21214-21219

7. Jiang, Y.; Yao, M.; Feng, J.; Niu, H.; Qiao, B.; Li, B.; Wang, B.*; Xiao, W.*; Dong, M.*, Yuan, Y. Molecular Insights into Converting Hydroxide Adenosyltransferase into Halogenase. J Agric Food Chem 2024, 72, 12685-12695

8. Yu, N.; Zhao H.; Wang, W.; Dong, M.*. Enzymatic fluoroethylation by a fluoroethyl selenium analogue of S-adenosylmethionine. ACS Catalysis. 2024, 14, 6211–6216

9. Jiang, Y.; Yao, M.; Niu, H.; Wang, W.; He, J.; Qiao, B.; Li, B.; Dong, M.*, Xiao, W.*; Yuan, Y. Enzyme Engineering Renders Chlorinase the Activity of Fluorinase. J Agric Food Chem 2024, 72, 1203-1212.

10. Wang, W.; Zhao H.; Yu, N.; Chen, F.; Dong, M.*. Stable S-Adenosylmethionine Analogue for Enzymatic Fluoromethylation. ACS Catalysis. 2023, 13, 13729-13734

11. Huang, R.; Zhi, N.; Yu, L.; Li, Y.; Wu, X.; He, J.; Zhu, H.; Qiao, J.; Liu, X.; Tian, C.; Wang, J *; Dong, M.* Genetically encoded photosensitizer protein reduces iron-sulfur clusters of radical SAM enzymes. ACS Catalysis, 2023, 13, 1240-1245.

12.  Zhi, N.; Zhu, H.; Qiao, J.; Dong, M.* Recent progress in radical SAM enzymes: New reactions and mechanisms. Chin. Sci. Bull 2022, 67, 1806-1820

13.  Li, Y.; Yao, Y.; Yu, L.; Tian, C.; Dong, M.* Mechanistic investigation of B12-independent glycerol dehydratase and its activating enzyme GD-AE. Chem. Commun., 2022, 58, 2738-2741

14.  Zhou, S.; Chen, X.; Ni, X*.; Liu, Y.; Zhang, H.; Dong, M.*; Xia, H*. Pyridoxal-5´-phosphate-dependent enzyme GenB3 Catalyzes C-3´,4´-dideoxygenation in gentamicin biosynthesis. Microb. Cell. Fact. 2021, 20:65, 1-12.

15.  Fenwick, M. K.; Su, Dan.; Dong, M.; Lin, H *; Ealick, S. E.* Structural Basis of the Substrate Selectivity of Viperin. Biochemistry 2020, 59, 652–662.

16.  Dong M, Dando EE, Kotliar I, Su X, Dzikovski B, Freed JH, Lin H. The asymmetric function of Dph1-Dph2 heterodimer in diphthamide biosynthesis. J. Biol. Inorg. Chem. 2019, 24, 777-782.

17.  Fenwick, M. K.; Dong, M.; Lin, H.; Ealick, S. E.*. The Crystal Structure of Dph2 in Complex with Elongation Factor 2 Reveals the Structural Basis for the First Step of Diphthamide Biosynthesis. Biochemistry 2019, 58, 4343–4351.

18. Dong, M.; Kathiresan, V.; Fenwick, M. K.; Torelli, A. T.; Zhang, Y.; Caranto, J. D; Dzikovski, B.; Sharma, A.; Lancaster, K. M.; Freed, J. H.; Ealick, S. E.; Hoffman, B. M.; Lin, H. Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis. Science 2018. 359, 1247-1250.

19. Dong, M.; Horitani, M.; Dzikovski, B.; Freed, J. H.; Ealick, S. E.; Hoffman, B. M.; Lin, H. Substrate-Dependent Cleavage Site Selection by Unconventional Radical S-Adenosylmethionine  Enzymes in Diphthamide Biosynthesis. J. Am. Chem. Soc. 2017. 139, 5680-5683

20. Dong, M.; Horitani, M.; Dzikovski, B.; Pandelia, M. E.; Krebs, C.; Freed, J. H.; Hoffman, B.; Lin, H. Organometallic complex formed by an unconventional radical SAM enzyme. J. Am. Chem. Soc. 2016. 138, 9755-9758.